Kritski AL, Conde MB, Muzy de Souza GR: Tuberculose do Ambulatório a Enfermaria. PubMed Int J Tuberc Lung Dis. Kivihya-Ndugga L, van Cleeff M, Juma E, Kimwomi J, Githui W, Oskam L, Schuitema A, van Soolingen D, Nganga L, Kibuga D, et al: Comparison of PCR with the routine procedure for diagnosis of tuberculosis in a population with high prevalences of tuberculosis and human immunodeficiency virus. However, inter laboratory and cost-effectiveness studies in other settings are required to evaluate the performance of the PCR dot-blot under field conditions before it may be introduced for routine use. Catanzaro A, Perry S, Clarridge JE, Dunbar S, Goodnight-White S, LoBue PA, Peter C, Pfyffer GE, Sierra MF, Weber R, et al: The role of clinical suspicion in evaluating a new diagnostic test for active tuberculosis: results of a multicenter prospective trial. BMC Public Health. The proportion of inhibitors was 1.9% for the in house PCR, similar to those used in NAA tests (0.85% to 22.7%) [12, 28]. 2003, 41 (3): 948-953. Tuberculose ICBd: Diretrizes Brasileiras para Tuberculose. In-house PCR results used in this study are not necessarily generalizable to other situations, as in house PCR are not standardized and is usually associated with low reproducibility, unless the replicating sites use the exact same in-house PCR [5]. Tuberculosis or Nocardia depending on full or partial retention of the dye. The sensitivity analysis, comparing the cost-effectiveness of AFB smear plus culture and AFB smear plus PCR dot-blot, and adjusting different scenarios for TB prevalence, shows that the ratio of cost-effectiveness remains more or less the same in most adjustments. 1996, 51 (3): 320-322. A decision analytic model was developed using the Excel 7.0 software to estimate the costs of the routine diagnostic procedures for diagnosis of PTB using sputum specimens: 1) AFB smear used with culture, and 2) AFB smear used with PCR dot blot. you with a sample collection cup. The total screening costs are 3.8 times for AFB smear plus culture versus for AFB smear plus PCR dot blot costs (US$ 5,635,760 versus US$ 1,498, 660). You may be asked to deliver one or both of these samples. The cost of treatment of false negatives cases was more cost-effective for the AFB smear plus PCR dot blot strategy than the AFB smear plus culture strategy (US$ 25,382 versus US$ 72,675). Coughing up blood. The tuberculosis infection is caused by the Mycobacterium tuberculosis AFB. AFB testing can help diagnose, track, and minimize the spread of TB in these populations and help determine the effectiveness of treatment. Jama. Eur Respir J. 2005, 3, The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/9/216/prepub, We thank laboratory staff from CDCT and FEPPS-LACEN; CEARGS (Center of studiesin AIDS/RS) for statistical support; and health personnel from SPRH. 2006, 54: 219-234. Sarmiento OL, Weigle KA, Alexander J, Weber DJ, Miller WC: Assessment by meta-analysis of PCR for diagnosis of smear-negative pulmonary tuberculosis. However, the cost of treatment of false positives cases was less cost-effective for the AFB smear plus PCR dot blot strategy than the AFB smear plus culture strategy (US$ 18,674 versus US$ 1,589) for outpatients. Laboratory technicians and respiratory specialists were blinded for the results of cultures and PCR dot-blot, and for the chest radiographs results and clinical predictors, respectively. Besides, individuals who are already undergoing treatment for Tuberculosis, are frequently asked to undergo this AFB test to determine how well the body is responding to the specific therapy. Also known as - Acid-Fast Bacillus Culture . Schijman AG, Losso MH, Montoto M, Saez CB, Smayevsky J, Benetucci JA: Prospective evaluation of in-house polymerase chain reaction for diagnosis of mycobacterial diseases in patients with HIV infection and lung infiltrates. 10.1128/JCM.43.6.2563-2566.2005.
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