AFB tests look for AFB bacteria in sputum. Acid-fast bacillus (AFB) is a type of bacteria that causes tuberculosis (TB) and other infections. To evaluate the efficacy of three sputum acid-fast bacillus (AFB) smears to rule out pulmonary tuberculosis, sputum AFB smear and culture results were analyzed at two university-affiliated teaching hospitals. The AFB culture is the inoculation of a clinical specimen onto culture media Becton-Dickinson Mycobacteria Growth Indicator Tube (B-D MGIT broth) and Lowenstein-Jensen (L-J) media slant, incubation at 37°C for up to six (6) weeks and detection of growth or no growth during this incubation period. AFB smear refers to the microscopic examination of a fluorochrome stain of a clinical specimen. However, a culture must be performed to confirm a diagnosis and identify the species of mycobacteria present. For people with signs and symptoms of an active TB infection, AFB smear results are considered together with results from NAAT for TB, as recommended by the Centers for Disease Control and Prevention. 3+ No AFB per 100 fields 1 - 9 AFB per 100 fields* 10 - 99 AFB per 100 fields 1 - 10 AFB per field ... Probability of a positive ... of bacilli/ml sputum # of bacilli observed Toman K. Tuberculosis Case Finding and Chemotherapy. Learn more. An AFB smear test gives results in 1–2 days but can't confirm a diagnosis. A sputum stain for Mycobacteria is a laboratory test performed on a sample of your sputum, or phlegm. Questions and Answers. An AFB culture can confirm a diagnosis, but results take 6–8 weeks. It’s also known as an acid-fast bacillus (AFB) stain or a tuberculosis (TB) smear. Positive AFB smears indicate a probable mycobacterial infection.
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