Once the sputum (phlegm) is in your mouth, release it into the container provided by the study nurse. Transtracheal aspirate Collect washing or aspirate in sputum trap Place the brush in a sterile, leak- proof container with up to 5 ml of sterile saline Minimum volume is 3 ml 50-ml conical tube or other sterile container Transport as soon as possible at room temperature If transport is delayed more than 1 hour, refrigerate specimen. 7. • Use liquified sample for downstream nucleic acid extraction following the extraction system manufacturer’s guidelines. The study nurse should hold the container up to the light to make sure you have provided a quality sample. To collect a sputum sample, you should do the following: Brush your teeth and rinse your mouth without using antiseptic mouthwash. Check– Give the sputum (phlegm) container to the study nurse to check for quality. Discard any unused reconstituted DTT. The use of a combination of chemical and enzyme-based lysis increases DNA recovery from sputum samples. If you’re having trouble coughing up enough sputum, your doctor may try tapping on your chest to loosen the sputum. Use to obtain samples in children, who do not produce sputum when they cough. A sputum culture is a sample of the gooey substance that often comes up from your chest when you have an infection in your lungs ... or having a collapsed lung during this process. Take a couple of long, deep breaths. • Incubate sample at room temperature with intermittent mixing until the sample is liquified (up to 30 minutes). They may also have you inhale a steam-like mist to help you cough up the sample. sputum + 500 µL of diluted DTT). Box 1 provides a reminder of the general principle of spe… Tube is inserted through the patient’s mouth or nose and passed into the stomach to get a sample of the gastric secretions that contain sputum that has been coughed into the throat and then swallowed. Specimens must be collected at the right time, using the correct technique and equipment, and be delivered to the laboratory in a timely manner (Dougherty and Lister, 2015). Testing the Sample. Obtaining a specimen involves collecting tissue or fluids for laboratory analysis or near-patient testing, and may be a first step in determining a diagnosis and treatment (Dougherty and Lister, 2015). Breathe deeply again and cough hard until sputum comes up. Gastric Washing. Note: Use DTT immediately. Spit out the sputum into the sample cup. This method reduces the possibility of losing material after lysis because the whole of the lysate from samples is transferred into the DNA purification column. Repeat– On day 1, you need to produce two high 8.
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