Using this model, the predicted value of response were find out within the domain of limiting values of independent variables. Effect of growth conditions of Lactobacillus gasseri OLL2809 on the immunostimulatory activity for production of interleukin-12 (p70) by murine splenocytes. temperature, pH, volume of inoculum, age of inoculum and additional carbon and nitrogen sources were selected for the growth of L. acidophilus. [ Links ], Das H. Empirical model development. Int Dairy J. 2019 Oct;13(3):178-183. doi: 10.22074/ijfs.2019.5584. Optimizing conditions for the growth of Lactobacillus casei YIT 9018 in tryptone-yeast extract-glucose medium by using response surface methodology. Oral administration of heat-killed Lactobacillus gasseri OLL2809 reduces cedar pollen antigen-induced peritoneal eosinophilia in Mice. Lactobacilli [sing: lactobacillus] are a rod-shaped, Gram-positive, fermentative, facultative anaerobic or microaerophilic organotrophs. In most cases they form chains of varying length. Therefore, it was deduced that the integrity of the bacterial cells is necessary for the stimulatory effect on IL-12 (p70) production and that acidic pH and heat treatment contributed to the stimulation by inhibiting the activity of autolysins indigenous to the bacteria. Braz J Chem Eng. 2008). [ Links ], Negi S, Banerjee R. Optimization of extraction and purification of glucoamylase produced by Aspergillus awamori in solid state fermentation. ANN is computational models of nervous systems. Khodaverdi S, Mohammadbeigi R, Khaledi M, Mesdaghinia L, Sharifzadeh F, Nasiripour S, Gorginzadeh M. Int J Fertil Steril. 5). [ Links ], Sanders ME. Key words: Response surface methodology (RSM), Artificial neural network (ANN), Genetic algorithms (GA), Box-behnken besign (BBD). Further, they secrete by-products such as lactic acid and acetic acid, which lower the pH, thereby creating a hostile environment for the growth of pathogenic microorganism. 2006 Aug;89(8):2846-55. doi: 10.3168/jds.S0022-0302(06)72557-7. Probiotic bacteria such as Lactobacillus and Bifidobacterium sp can play an important role in promoting human health in the gastrointestinal tract (Mitsuoka 1990). Table 5 showed the optimum value, or combination of different process parameters on which the bacterial growth measured by optical density (OD) was maximum for L. acidophilus. For the different growth parameters it was desired to develop a polynomial model between the Lactobacillus growth and growth parameters to develop the following relationship between the coded values x1, x2, x3, x4, x5 and x6 of independent variables and dependent variable Yp as shown below. Hoboken: Wiley-Interscience; 2002. Normally, they form straight rods but under certain conditions spiral or coccobacillary forms have been observed. Currently, consumers are very much concerned about the nutritional and functional attributes of food worldwide. Nutrients. [ Links ]. Endocr Metab Immune Disord Drug Targets. Functional cultures and health benefits. In addition, the observations of a difference in the stimulation of IL-12 (p70) production by L. gasseri OLL2809 grown under various conditions are consistent with the characteristics of autolysis. Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens. They actively contribute in the digestion, immune stimulation and inhibition of pathogens such as Bacteroides, Escherichia, Clostriduim and Proteus, which are potentially harmful bacteria found in the gastrointestinal tract (Ziemer and Gibson 1998). 2005; 43: 38-43. Braz Arch Biol Technol. The world probiotic market is estimated at $15 billions, which included 10% of the lactic bacteria drink market. Genomic and phenotypic evidence for probiotic influences of Lactobacillus gasseri on human health. It started with two neurons and was increased up to six. Consequently, the global functional food market is thriving with recent estimates indicating up to a $50 billion annual share. The culture was maintained at 4°C and sub-cultured on the slants of MRS (De Man Rogosa and Sharpe) growth medium two times in a month. 30oC, pH 6.5) with an optical density around (1.9297) at 540 nm, while the lowest growth rate was at (Temp. 2009; 2: 82-92. 1992; 7: 357-413. [ Links ], Kumari KS, Babu IS, Rao GH. For our investigation we used thirty Lactobacillus-slrams and four strains belonging to other genera, but known as common brewery infectants. 2019 Feb 2;11(2):325. doi: 10.3390/nu11020325. J Ind Microbiol. 2001; 73: 361-364. Process optimization through statistical method is a technique in which changes or adjustments are made in a process to get better results (Myers and Montgomery 2002). eCollection 2017. This study developed the empirical model to increase the cell growth of L. acidophilus by optimizing growth parameters such as temperature, pH, inoculum volume, incubation period and additional effect of different carbon and nitrogen sources using the RSM, ANN and GA. All the solvents and reagents used in the present study were procured from Merck, Germany.
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