This vision system process four sputum image for each sputum sample and determine the average number of pus cells and epithelial cells in that sample. In either case, a minimum of 20 LPFs should be observed. By continuing you agree to the use of cookies. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Can sputum gram stain be used to predict lower respiratory tract infection and guide empiric antimicrobial treatment: Experience from a tertiary care hospital, https://doi.org/10.1016/j.mimet.2019.105731. Good sputum samples depend on thorough healthcare 1 0 obj <>]/PageLabels 6 0 R/Pages 3 0 R/Type/Catalog/ViewerPreferences<>>> endobj 2 0 obj <>stream Sputum gram stain grade는 다음과 같습니다. 1 이 검체에서 전형적으로 배양이 이루어집니다. The present study was conducted on 1000 respiratory specimens which were processed using conventional microbiological techniques. %PDF-1.6 %���� However, the final grading of either the sputum sample is positive or negative is 100% accurate. The sensitivity for predicting infection was higher for good quality sputum samples (54%) as compared to poor quality sputum samples (37%). ��䭵kw��72����z~P�b("W&1PR�zQ�}1�ݡ�3*g3���9��W L(��Ġ�^'o�g�}�. xmp.did:36DAE03747B6E211826DC0B6B1078A96 This system also able to determine either the sputum sample is positive or negative. Gram stain smear and culture results were compared statistically to assess the sensitivity, specificity, positive and negative predictive value. / uuid:11b54849-05f9-43cd-8c8c-49b48b6d2af8 2014-04-15T15:58:05+05:30 This specimen would be considered unacceptable for culture. Adobe InDesign CS6 (Windows) Prior to culturing a sputum specimen, a Gram stain should be performed to evaluate the quality of the specimen. grade indicates relative bacterial burden and correlates with disease presentation. Potential pathogens were obtained from 28 of 209 deeply coughed out sputum samples (13.3%) and from 19 of 315 saliva mixed sputum samples (6%). One of two criteria are currently used to determine if the specimen is contaminated with oral flora organisms, which would make the specimen unsuitable for culture. )ى�ŢudS�px�̙�O>�Iz?�(�K�:��t׎~��y����0����4Kh���� ���� ?��l�it4�ox��ѫ��7�ߎ��)��L�$�{2�i�]��ώ� �tv��'�_G��x�8��&&N�:2�w3���\�!��ș�:V��.�����������ǟ�z��O���!/��̗#L� 59.indd In absence of these supportive measures smear examination should not be relied upon for empiric treatment. Prior to culturing a sputum specimen, a Gram stain should be performed to evaluate the quality of the specimen. 즉 grade 5가 이상적인 객담 샘플입니다. One of two criteria are currently used to determine if the specimen is contaminated with oral flora organisms, which would make the specimen unsuitable for culture. 2014-04-15T15:58:05+05:30 If the specimen is determined to be a good quality lower respiratory tract specimen, continue to examine the slide under oil immersion (1000X) magnification for bacteria, yeast, and polymorphonuclear white blood cells (PMNs) and proceed with culturing the specimen. Background: The usefulness of sputum culture in guiding microbiological diagnosis of community-acquired pneumonia is controversial. Aim: This study aimed to determine the diagnostic value of sputum quality assessment about sputum culture for diagnosing LRTIs in children. Review and follow your laboratory's criteria. All samples, regardless of their quality, were cultured, identified, and antimicrobial susceptibility testing was performed by Kirby-Bauer disc-diffusion method.

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